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The 14 complete elements were checked for the presence of target site duplications (TSD): all but one have TSDs.
Double mutants were confirmed by non-complementation with caps and trn alleles and the exact deletion sites induced by the P-element were checked by inverse PCR and sequencing.
Fungal elements were checked for a non-specific fluorescence signal outside of the window of the probes used.
The different elements were checked by the appropriate health-care professionals, who signed the corresponding section after completing the verification process.
After initial annotation quality assurance of the database was performed by re-review of the entire dataset with the literature, and the data elements were checked for duplications and errors.
The reproducibility (± 5% for most elements) was checked by digesting one sample in triplicate.
Results obtained from the cost-effective approach with two-dimensional COMU elements are checked against experimental data [Frew DJ, Forrrestal MJ, Hanchak SJ. Penetration experiments with limestone targets and ogive-nose steel projectiles.
The suited thermal behaviour of the component was checked using finite element modelling.
Prior annotation of the predicted Ds elements and of the genome sequences 200 bp upstream and downstream from the element's insertion site were checked in the database at maizesequence.org.org
A library containing all piggyBac elements identified in M. murinus were checked against RepBase to determine similarity to other known elements.
The potential of the new element and the control technique were checked in simulations including sphere fracture in composites made up of random distribution of elastic spheres within an elasto-plastic matrix.
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