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LOD for each element was determined from the standard deviations and calculated based on twelve blank measurements.
The position of the UY element was determined by sequencing the flanking DNA obtained by inverse PCR, according to the Berkeley Drosophila Genome Project (BDGP) protocol.
PCR was conducted under standard conditions and the presence or absence of the Doc element was determined by the size of the amplified fragment (5.5 kb with the insertion, 1 kb without) as determined by 1% agarose gel electrophoresis.
The expected number of each element was determined by calculating the number of elements that would be present in the region in relation to its size if the elements inserted randomly throughout the genome.
Assembly was performed by the program Velvet [ 47] using a hash_length of 50 and –ins_length of 480, and the identity of the inserted element was determined from the resulting contigs by RepeatMasker (http://www.repeatmasker.org).org
The age of each element was determined by aligning the paired-end sequences and converting the observed sequence divergence to an estimated number of substitutions per site by the Jukes Cantor method (Jukes and Cantor 1969).
Similar(54)
Secondly, the deflections and velocities at the stations, containing non-linear element, are determined by iteration.
Then, the stiffness and mass matrices of the last shaft element are determined by means of the Lagrange's equations.
The steel concrete interface was idealized by a number of interface elements and the properties of interface element were determined as the results of push-out tests.
The thermo-dependent constitutive behavior of each cohesive element is determined through finite element (FE) simulations of its corresponding potential damage zone at different elevated temperatures.
From the above analysis, the value of each element is determined.
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