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Glasmacher, E. et al. A genomic regulatory element that directs assembly and function of immune-specific AP-1-IRF complexes.
Musatov, S., Roberts, J., Pfaff, D. & Kaplitt, M. A cis-acting element that directs circular adeno-associated virus replication and packaging.
Glasmacher, E, Agrawal, S, Chang, AB, Murphy, TL, Zeng, W, Vander Lugt, B, Khan, AA, Ciofani, M, Spooner, CJ, Rutz, S, Hackney, J, Nurieva, R, Escalante, CR, Ouyang, W, Littman, DR, Murphy, KM, and Singh, H. "A genomic regulatory element that directs assembly and function of immune-specific AP-1-IRF complexes". Science 338, no.
The c- fos mRNA mCRD (major coding-region determinant) is an 87 nt G/A-rich element that directs translationally coupled deadenylation.
This process is due to the presence of a cis-acting element that directs protein translation to continue beyond the canonical stop codon, with translation stopping at an alternative downstream stop codon.
To determine whether neoislets were derived from intestinal epithelial cells, we generated a panel of transgenic mouse lines that express rtTA*M2 under control of the Villin promoter (Villin-rtTA*M2), a well-characterized promoter element that directs broad expression within the intestinal epithelium (el Marjou et al., 2004; Madison et al., 2002; Pinto et al., 1999).
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The screen also identified other cis-acting elements that directed expression in the central complex.
Histone modifications are important markers of function and chromatin state, yet the DNA sequence elements that direct them to specific genomic locations are poorly understood.
Mimicking this strategy requires the design of a set of multifaceted molecules, where elements that direct assembly were conjugated to biologically specific components.
In terms of transcriptional regulation of mouse Nkx3.1, a 5-kb fragment downstream of the Nkx3.1 coding region was shown to contain elements that support expression in the prostate and bulbourethral glands, whereas an upstream fragment was found to contain elements that direct expression in somites and testes [61].
In addition, the TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1-20 (TCP20) TF was recently identified as a key element in the systemic signaling pathway that directs N foraging in Arabidopsis roots, thus opening up the possibility of controlling root plasticity to improve soil exploration capacity in crops.
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