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The Neural Network Promoter Prediction v.2.2 [68], TRES – Transcription Regulatory Element Search, which performs searches within the TRANSFAC database [69] and the PromScan program, that generates an alignment of known sequences and matrix frequency [70], were used to scan DNA sequences for potential binding sites.
The 'Single-platform Microarray Element Search' and the 'Multiple-platform Microarray Element' search tools allow users to identify the specific platform probes that map to a common gene target.
In our in silico analysis, 700 bp 5' upstream regions of the NTH1, TPS1 and YLR270w genes were screened using the MatInspector v2.2 [ 13], FastM [ 14], TRES (Transcription Regulatory Element Search) [ 15], and SCPD (Saccharomyces cerevisiae Promoter Database) [ 16] algorithms searching for transcription factors motifs and consensus sequences annotated on the Transfac 5.0 database [ 17].
Transcription element search software (TESS) sequence analysis [29], [30] of 4 Kb of the 5'-flanking region, exon 1 and the beginning of the first intron of the human COQ7 gene revealed two potential binding sites for NF-κB, three for Sp1, as well as one for RXR (figure 3).
To characterize the PP2A-Aα gene promoter, we have analyzed the proximal promoter sequence using web software TFSEARCH (transcriptional factor search) and TESS (transcription element search system), and found four cis-elements with the highest scores for CREB, ETS-1, SP-1 and AP-2α in this region (Fig. 2A).
Reference-based repeat element search was done using the Repbase libraries v20130422 [ 56].
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The N2pc is thought to reflect visual-spatial attention mechanisms that separate relevant and irrelevant perceptual information in bilateral, multi-element search arrays (a mechanism hereafter referred to as visual selection).
The cis-element search results were implemented into the SoybeanTFDB as a searchable property.
The cis-element search results were implemented into the GraminaeTFDB as a searchable property.
The cis-element search results were implemented into the TreeTFDB as a searchable property.
> Out of 873 URDEGs, 680 were found in the Osiris promoter database and subjected to cis-element search.
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