Several criteria were used before a sequence was included in our set of electronically mapped sequences.
While bacteriophage Qβ can evolve extremely quickly, we believe that our sequences represent the first complete, accurate, and electronically accessible sequence of the bacteriophage genome, and the first detailed examination of the genome of a Qβ quasi-species.
However, to conveniently manage these molecules electronically, the sequences are usually displayed in linear form by opening the circular DNA at a specific position where the base is either numbered 1 (if located downstream) or the final base (if it is located upstream).
We demonstrate the value of proteomics to experimentally support the in-vivo expression of electronically predicted proteins of a newly sequenced genome.
Grape (Vitis vinifera), has only recently been sequenced and most genes are electronically inferred.
Recombinant clones were sequenced using Big Dye Terminator chemistry and ABI 3730xl sequencers (Applied Biosystems, CA), and vector and low-quality sequences were electronically trimmed.
We contributed to the annotation of the newly sequenced bovine genome by experimentally confirming the in vivo expression of 4,313 electronically predicted proteins (see additional file 1).
Instead, a series of computer-controlled antennas -- the phased array -- are electronically switched in sequence to steer the radar signals in a scanning motion.
He got the effect he wanted by sliding metal against wood and having a melon drop into a basket, then electronically magnifying the sequence.
In particular, genes with repeat-rich or modular elements, like dystrophin and collagen 6A3, may be more difficult to align electronically, resulting in genomic sequences that do not agree with BAC sequence data.
