The intracellular muscle recordings were performed using a BRAMP-01R amplifier (npi electronic GmbH).
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Finally, a colloidal cellulose preparation was obtained by repeated 1 min long sonification (Sonoplus; Bandelin electronic GmbH & Co. KG, Berlin, Germany) of the suspension cooled on ice.
A tubing warmer provided additional temperature control to inflowing medium immediately before the chamber (AstothermR Plus 220/220 S, Stihler Electronic GmbH, Stuttgart, Germany; Astotube cat #IFT30410 dimensions 6.8mm OD).
About Sennheiser Communications Sennheiser Communications is the result of a joint venture between the electro acoustics specialist Sennheiser electronic GmbH & Co. KG and the hearing healthcare specialist William Demant Holding Group.
Skin pH was measured with a skin pH meter (PH905, Courage-Khazaka electronic GmbH, Cologne, Germany) because the acid mantles in the skin play the role of antibiotic defensive barriers.
The resultant mixture was sonicated for 5 h using Sonorex Super RK 255 H ultrasonic bath from Bandelin (Bandelin Electronic GmbH & Co. KG, Berlin, Germany), operated at a frequency of 35 KHz and an effective power of 160 W.
Skin temperature was also measured with a skin thermometer (ST500, Courage-Khazaka electronic GmbH, Cologne, Germany), which is a device used to measure skin temperature by sensing infrared rays emitted from the atopic dermatitis areas of the skin.
Determination of the persistent current fraction was done in Xenopus laevis oocytes using the two-microelectrode voltage clamp technique using a commercial amplifier (TEC-05-S; npi electronic GmbH, Tamm, Germany), as previously described [29], [36].
Skin samples were collected using Corneofix® (Courage and Khazaka Electronic GmbH, Cologne, Germany) foils.
