TEM images showed electron dense areas at the poles of the cells, which is typical of protein based inclusion bodies found in the cytoplasm of E. coli cells (Fig. 3D).
As attenuation for electron dense areas at CT energies (up to 140 keV) is typically an order of magnitude higher than attenuation at the PET photon energy (511 keV), an overestimation of PET activity can be expected in those areas after CT-based attenuation correction.
Their secretion is granular, but shows a characteristic structure with an inner, electron-dense area in a non-electron-dense oval structure (Fig. 7a, b).
In the corresponding area of a mature flagellum, the basal plate is a loose electron-dense area in which one, sometimes two, open or closed CP minus ends are found.
Americium is a heavy metal; thus, its accumulations in the ultrathin sections would appear as electron-dense areas.
As seen in Fig. 2A, our analysis indicates that in transformed Hfq− bacterial cells induced with 0.001%, Hfq-MT accumulates in internal electron-dense areas of the bacteria, compatible with the location in the nucleoid reported before [21].
About 80%% of cells population, showed cell wall disruption and electron-dense areas of amorphous material packed with crushed microsomes and ribosomes.
On early endosomes, Hrs and clathrin form dynamic microdomains, which can be seen by EM as flat electron-dense areas on the limiting membrane (Raiborg et al. 2001; Sachse et al. 2002).
TEM showed electron-dense areas in the polyplexes, which could be the siRNA, and the presence of very small particles (about 40 nm) in all polyplex formulations besides larger particles of 440, 330, and 189 nm for linear bisspermine, linear tetraspermine, and dendritic tetraspermine polyplexes, respectively.
In order to quantify the labelling density and its variations in the different nuclear domains, the primary antibody was immuno-gold labelled and the sections were observed by electron microscopy to determine the number of electron dense particles per surface area (Figure 3D F).
