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For economic reasons, the electrodes were in the form of sputtered films on glass discs which could be mounted on the shaft of a rotating disc electrode apparatus.
In this paper we present the electrode apparatus and methods, as well as the characterization results, e.g. signal vs. time and induction factor, of such chips and discussing the highlight and problems of this new concept.
We present the electrode apparatus and methods and biochip characterization of such chips, e.g. signal vs. time and induction factor, and discuss the advantage and potential problems of the new biofilm-biochip technology.
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Kinetic activity was measured ex situ by LSV in rotating disk electrode (RDE) apparatus with 5 mV/s sweep rate and 1,600 rpm speed of the rotating disk.
Kinetic activity for ORR is measured ex situ with Linear scan voltammetry (LSV) in rotating disk electrode (RDE) apparatus with 1600 rpm rotating rate and 5 mVsweepweep rate in O2 purged 0.5 M HClO4 solution.
Because of the incorporation of a drive mechanism in the electrode array apparatus used for one monkey, we were able to advance the electrodes by ∼100 microns when an unstable period occurred, after which we often entered another stable period of recording.
ECGs were recorded from the surface of the heart by electrodes constructed from 2 mm Ag/AgCl pellet electrodes (Harvard apparatus, UK).
The 5 electrodes of this apparatus were connected subcutaneously at the limbs.
The device is ready to use that does not need any extra mechanical apparatus for electrode insertion.
After catheterization and placement in the stereotaxic apparatus, a stimulating electrode was lowered into the VTA.
The sockets were fitted into a 6-pin electrode pedestal and the entire apparatus was secured with dental cement (Stoelting).
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