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Exact(12)
Group 3 consisted of 22 C55 and C65 variants in the FAT and kinase domains [ 16], as well as protein-truncating variants (either splice, nonsense, or frameshift).
However, this antibody did not immunoprecipitate either splice variant, and moreover it did not show sufficient specificity or affinity to recognise unambiguously by Western blotting the endogenous proteins in tissue extracts of kidney or pituitary.
A single hydrogen or O-methyl group was substituted for the 2'-hydroxyl group at either splice site of a nuclear pre-messenger RNA substrate.
RT-PCR was performed using PMCA isoform-specific primers designed to amplify transcripts that included either splice site A or splice sites B and C. PMCA cDNAs were sequenced or cloned, and then sequenced.
They either splice audio ads between songs or DJ chatter, post click-through banner ads on the site or offer sponsorships so that a travel agent specializing in Caribbean vacations, for example, could attach itself to a reggae music channel.
Splicing of the modified pre-messenger RNA's in vitro revealed that, although a 2'-hydroxyl is not absolutely required at either splice site, the 2'-hydroxyl at the 3' splice site is important for the second step of splicing.
Similar(48)
Either spliced "sushi-only" or shed sIL-15Rα is absolutely required for IL-15 secretion.
A set of RNAs, either spliced or full genome length, is transported from the nucleus to the cytoplasm, where RNAs can be translated or packaged.
For each unique Difference Region for which there was at least one supporting EST, we tested the null hypothesis that there is no bias for or against either variant form, i.e., either splicing alternative was equally likely.
DOI: http://dx.doi.org/10.7554/eLife.03656.002 Intron-containing RNA transcripts are usually retained in the nucleus until they are either spliced or degraded (Luo and Reed, 1999; Bousquet-Antonelli et al., 2000; Zhou et al., 2000).
As such, small-scale mutations may either disrupt splicing signals or create cryptic splicing signals, leading to changes in splicing patterns, converting formerly intronic sequence into exonic sequence or vice versa [ 19- 22].
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