In brief, whole female flies were placed in empty vials for 30 minutes (min), then transferred to Eppendorf tubes in batches of 12, weighed, and either processed immediately or stored at −80 °C for later processing.
The heating oil is used in the boilers and the glycerol is either processed with the food waste or sold to the cosmetics industry to create beauty products.
Blood was then either processed immediately or stored undisturbed in 4 °C or RT (20 25 °C), protected from light, for 24 72 h.
Strippings were either processed for standard electron microscopy (EM) or for ruthenium-, osmium-tetroxideide vapor fixation, followed by immediate epoxy embedment, an artifact-free protocol, which, to our knowledge, is previously unreported.
For comparison, EPD-formed green bodies were either processed directly to ceramic bodies or after additional compacting by hot-pressing in a piston-cylinder apparatus.
After purification, T cells were either processed directly to measure intracellular lactate concentration or cultured in complete culture medium, with or without stimulation (see below).
At this stage, the extract was either processed further or stored at −20°C.
Samples were either processed immediately or stored in 2 ml tubes over night at −20°C prior to being processed.
Fish were removed from gill nets and were either processed immediately on the boat or placed in floating mesh pens along side the boat until being processed.
Frames were extracted and either processed using a custom ImageJ script [32] or analyzed with a custom script for Matlab (The MathWorks) [5].
The TG were rapidly removed and either processed for Chromatin ImmunoPrecipitation (ChIP) assays or placed in RNAlater (Ambion, Inc., Austin, TX) for RNA extraction.
