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When PGG2 was used as substrate (as shown in Figure 5C and 5F), myricetin stimulated the catalytic activity of COX I and II that were either pretreated with aspirin or received no pretreatment to a similar extent.
4-week-old plants were either pretreated with H2O or 1 µM chitooctaose 24 hr before inoculation with P. syringae.
HUVECs were either pretreated with or without 3-MA before the addition of 1 μM METH for 72 h.
The analysis was performed using data from 108 patients from a phase II clinical trial investigating the efficacy of eribulin mesilate in CRPC patients who were either pretreated with docetaxel- (n = 50) or taxane-naïve (n = 58).
For the unprimed conformation, samples were either pretreated with apyrase (10 U/ml for 30 min or 24 hr on ice) to remove ADP and ATP, or used directly after dilution (all ATP in the PMEG buffer had been hydrolyzed to ADP, as confirmed by HPLC [data not shown], giving a final ADP concentration of 4 666 μM).
Acute inflammation in the peritoneal cavity was induced by an i.p. injection of recombinant mouse TNFα (5 µg/mouse in a final volume of 100 µl) into mice either pretreated with siRNA or saline control.
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SurePath samples were either pretreated manually with DNA denatured prior to testing according to the manufacturer's protocol, or DNA was isolated and purified using the DSP AXpH DNA kit on the QIASymphony SP platform (Qiagen, Hilden, Germany).
Monolayers were either pretreated (1 h) with blockers, and then incubated with cytokines (24 h), or treated without test agents and then treated with cytokines (24 h).
To measure NF-kB activation, monolayers were either pretreated (1 h) with melatonin, and then co-treated with TNF-a (30 min), or treated without test agents and co-treated with TNF-a (30 min), or not treated (controls).
In BMMC from TLR4 knockout mice, we did not detect production of TNFα or IL-6 in response to a second challenge with LPS, in cells that were either pretreated or not treated with LPS.
They were then either untreated or pretreated with 40 n VCR (Sigma-Aldrich) for 1 h or pretreated for 24 h with either 5 μ suberoylanilide hydroxamic acid (SAHA Merck & Co. Inc.., Whitehouse Station, NJ, USA), 0.5 μ 17-allylamino-17-demethoxy-geldanamycin 17-allylamino-17-demethoxy-geldanamycin 17-allylamino-17-demethoxy-geldanamycin 17-allylamino-17-demethoxy-geldanamycin 17-allylamino-17-demethoxy-geldanamycin
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com