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It appears as though either orientation of Met31 can be used within the docking complex (Fig. 2).
However, it is unclear whether either orientation of the ε-modified base can be efficiently utilized for nucleotide insertion.
However, because this method adds unique promoters to both the first and second cDNA strand (T7 and T3, respectively), either orientation of RNA can be produced from the same population of cDNA simply by selecting the appropriate RNA polymerase for IVT.
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Next, the FLP-inducible rep68 expression unit from pGS.pA+.Rep68 was inserted in either orientation upstream of the p5IEE in the dHV shuttle construct pAd/AAV.EGFP [14], hereafter referred to as pdHV, to generate pdHV.68/5'3' and pdHV.68/3'5', respectively (Fig. 1A).
IS orientation patterns were investigated for each chromosome and each IS family by scoring the number of IS elements having either orientation relative to the sense of movement of the replication fork as defined by the local GC skew sign to take into consideration possible recent chromosomal rearrangements.
They did not observe evidence for regular lattice structure in the arrangement of either orientation preference domains or pinwheel locations.
Deletion of the homologous recombination protein genes mitigated the effect of the srs2Δ mutation in either orientation, and decreased the effect of the sgs1Δ mutation in the (CTG 70 orientation, but exacerbated the effect of the sgs1Δ mutation in the (CAG 70 orientation [ 15].
Cloning of −8409/−7563-SV40Luc and −7563/−8409-SV40Luc were performed by digesting the original mouse genomic fragment by AflII/Eco47III and inserting the 846 bp fragment in either orientation in the SmaI site of the pGL3promoter vector (Promega).
This is because 1 and its known preferred binding site, DNA sequence 1, are both C2 symmetric, ensuring that either possible orientation of the intercalator initially associated with DNA will be aligned correctly to intercalate fully.
This observation emphasizes that the lack of electroactivity might be caused by either an inappropriate orientation of the protein (native cyt c) or a critical distance (MUA-cyt c).
After 500 ms midway through the counterphase cycle (when all elements reached mean luminance)—the target was switched for a Gabor that either matched the orientation of the flankers ("change-same") or was orthogonal to the flankers ("change-different").
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