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All experiments were performed using cells between the sixth and eighth passage.
MSCs between the fifth and eighth passage were used for the experiments.
Although the established cell line appeared to have epithelial cell morphology, we further investigated homogeneity at the eighth passage and also at the resuscitated cells by examining the protein expression of cytoskeleton 18, which is specific for epithelial cells, and vimentin which is usually expressed in various non-epithelial cells [23].
Chondrocytes were used for experiments up until the eighth passage.
Moreover, in NBSs, at the eighth passage, CD133 was markedly decreased (80 90%), whereas Oct4 did not change.
Although monolayer cultures were successfully maintained for up to 15 passages, T-CFU became undetectable by the eighth passage and remained so thereafter.
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BAECs were used between the sixth and eighth passages.
A second passage worth consideration is the goal itself.
the second passage.
Third to fifth passage cells were used for next experiments.
First passage time statistics, such as the survival probabilities and first passage time densities are obtained analytically.
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