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Thermal protein denaturation of egg samples was analyzed by differential scanning calorimetry (DSC) using the dynamic method, and estimated for several thermal schedules by kinetics analysis.
Additionally, DNA extracted from 25 fecal egg samples was also tested and the specificity of the assay was verified by sequencing the ITS-2 gene of all the Haemonchus positive and non-Haemonchus samples.
A first set of egg samples was collected from females undergoing natural (N) ovulation.
A third set of egg samples was collected from females subjected to a photoperiod manipulation (PM) of ovulation.
A second set of egg samples was collected from females subjected to a hormonal induction (HI) of ovulation.
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Over two sampling years, 75% (58/78) of the egg samples were infected with at least one virus whereof 32% (25/78) of the samples were infected with a single virus and 42% (33/78) were infected with multiple viruses.
Two hundred chicken egg samples were used for measuring freshness and spectra collection during egg storage (up to 25 days).
Performance was assessed during 4 weeks and feed and egg samples were collected in the last week of the experiment and analyzed for various variables with a focus on color and antioxidant traits.
All egg samples were immersed in their respective fixative for a minimum of three days before viewing.
For each bumble bee colony, five workers were tested upon arrival; for honey bee colonies, 20 workers and 10 egg samples were tested.
In 2014, a further 43 egg samples were obtained.
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