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Six 2 4 day old gravid females from each dfe line and six similarly-aged, non-virgin Canton-S competing females were placed in a vial for egg laying, with 10 vials (groups of flies) started for each genotype.
Flip-out was induced 24-48 hours after egg laying with 60 minutes heat shock at 37°C.
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Egg laying coincides with periods favourable for the development and emergence of hatchlings for instance, times of abundant food or of optimal weather conditions.
daGAL4 > UAS-InRDN females showed reduced egg laying compared with both daGAL/+ and UAS-InRDN/+ controls in both wild-type and dfoxo− genetic backgrounds (Fig. 4A).
But egg production is a complex trait because egg laying evolves with time, and it was only measured over a limited period in the works on the chicken, so the characters were not directly comparable.
Disruption of the egg-laying process produces an egl (egg laying abnormal) phenotype, with one class of egl mutation characterized by an increase in the number of fertilized eggs retained within the body and eggs being laid at a much later stage of development.
Similar to hst-3.1 and hst-3.2, kal-1 mutants do not show a defect in egg laying rate compared with wild-type control animals (data not shown).
For example a threshold model has been fitted in Bayesian analyses to data on fertility and other egg production traits in egg laying stock, but with the trait analysed solely as one of the female [ 16, 20].
The effect of mating treatment on female lifetime fecundity, mean egg weight (mg), mass of eggs laid (mg), and oviposition rate (eggs per day) in either of the four egg laying periods, together with means ± SE for each variable per treatment.
Secretions associated with egg laying include a pheromone to which adjacent individuals respond by extending the probosciform penis toward the source.
PRL was measured again during the birds' second, reproductively experienced, breeding cycle, beginning with egg laying until chick fledging.
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