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Egg incubation was in a humidified incubator at 37°C for 9-10 days with candling and manual turning of the egg thrice daily.
Egg incubation takes from 55 to 100 days.
The duration of egg incubation and pregnancy is temperature-dependent.
The duration of egg incubation varies from as little as nine days in some tropical perching birds to as long as 80 days in some albatrosses.
Egg incubation is performed by each parent in two or three long shifts over the course of the full 33 39-day period.
Investigations of thermoreception and thermoregulation in birds indicate that thermosensors exist not only in the skin on the body but also in the skin around the face, the thoracic brood patch (the area used for egg incubation), and the beak.
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On the 14th day of the eggs' incubation, the tumors were randomly divided into three groups: control group (C; non-treated tumors), AgNPs group (AgNPs; tumors treated with colloidal AgNPs), and placebo group (Pl; tumors supplemented with ultra-pure water).
LSCC implantation was performed on the 7th, 8th, or 9th day of eggs' incubation when the CAM is already formed.
On the 11th day of eggs' incubation, that is, after 48, 72, and 96 hours of tumor implantation, two eggs were reopened and live-fixed in the 10% formalin solution.
White et al. [295] Chickens 5, 20, 40 mg/kg egg; before incubation Impaired hatching rate, high prevalence of splayed legs, and chicks with partial or complete loss of yellow pigment in the down n.r.r
This sampling was done at three different times in each nest (egg laying, incubation and chick rearing).
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