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Cellular uptake and efflux on PC3 cells (n = 3).
The farming systems model, APSIM, was modified to include the effect of root citrate efflux on P availability in soil, crop P uptake and growth.
Two long-lasting P-gp pump modulators (9 and 10) were identified; they were able to inhibit remarkably the P-gp substrate rhodamine-123 efflux on the resistant K562/DOX cell line after 60 min.
The most potent compounds, 8, 9, 10 and 13, were further studied by evaluating their doxorubicin cytotoxicity enhancement (reversal fold, RF) and the inhibition of P-gp-mediated rhodamine-123 (Rhd 123) efflux on the K562/DOX cell line.
Accumulation of labelled chloramphenicol was monitored in the presence of an energy poison to determine the involvement of active efflux on the antibiotic intracellular concentrations.
However, the selectivity of solute efflux on which the αS pore hypothesis is primarily based, was measured in the presence of calcium [16].
Similar(42)
Compared with empty vector (pCMV6) control (EV), overexpression of STARD3 had no effect on efflux of [3H]cholesterol, under basal conditions or in the presence of HDL (50 μg/mL) (Fig. 1E); levels of efflux were comparable with wild type cells.
The capability of an influx transporter on one side and an efflux transporter on the other side of the cell to permit effective transcellular transport of the nutrients was revealed after identification of Lsi1 and Lsi2.
Within each trial, the dopaminergic efflux was switched on after a short interval from the trial onset and was switched off before its end.
Uptake studies performed on MRP4-enriched isolated membrane vesicles and efflux experiments on MRP4-transfected cells have made it possible to identify various physiological substrates for the transporter [88].
In the schematics of Figure 2, for clarity and convenience, we draw the peptide influx always on the left gate and the peptide efflux always on the right gate.
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