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We find the layered structure and growth advantage in downstream biofilms only when aggregates containing blue and yellow cells are preserved during effluent transfer.
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This loss in sensitivity is because under typical operating conditions the fraction of the first column (i.e., column 1) effluent transferred to the second column (i.e., column 2) is likely to be ∼5 10%.
Due to the high bio-refractory content of the wastewater, the effluent is transferred into an evaporation pond.
The process has an extraction sector where Cr VI) is extracted from the effluent and transferred to an organic solution and the stripping sector where Cr VI) is back-extracted from the organic solution into an aqueous phase and concentrated for recycle and reuse on-site.
The effluent was transferred back to the reactor for further fermentation.
The column effluent was transferred to a Megaflow electrospray probe of the mass spectrometer where the components were ionized and subsequently analyzed.
To measure the rate of incorporation of radiolabelled sugars, the column effluents were transferred in part (40%) to glass scintillation vials, evaporated to dryness under a stream of nitrogen, and dissolved in 10 ml Betafluor (National Diagnostics, Mannville, NJ, USA).
Nonetheless, the effectiveness of disinfection using ozone depends on the ozone dose, the ozone demand, the quality of the effluent and the transfer efficiency of the ozone system (Paraskeva and Graham 2002).
CZE was used as the first separation, from which the effluent components were transferred and further analyzed by MEKC.
The trap column effluent was then transferred to a reverse-phase microcapillary column (0.075 × 150 mm, Acclaim PepMap100 columnlumn, 3 μm, 100 Å; DIONEX, Sunnyvale, CA).
The trap column effluent was then transferred to a reverse-phase microcapillary column (0.1 × 150 mm, packed with Magic C18, 5 μm, 100 Å; Michrom Bioresources, Auburn, CA).
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