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Also, subsequent removal by flocculation and the possibility of better results by adaptation of microbes in textile effluent medium (where the carbon source is only in the form of effluent) has been observed (Pagga and Brown 1986).
Oppositely the R value for Neochloris sp. cultivated in the effluent was 0.819 and stated the high relevance of the effluent medium and incubation period provided in the respective change in neutral lipid content.
The changes before and after treatment with Neochloris sp. were measured; among four heavy metals that were presented in effluent medium only lead (Pb) was utilized by the strain.
The adhering cells were perifused with RPMI-1640 medium supplemented with 5% fetal bovine serum for 18 hr at the rate of 1 mL/hr, and the effluent medium was collected automatically in 2-mL aliquots.
The alveolar macrophages in culture were subjected to the presence of particulate (chrysotile asbestos) and soluble (phorbol myristate) toxicants, and their response was monitored in the effluent medium by measuring the release of prostaglandins (PGE) by radioimmunoassay.
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Al-Gheethi and Norli (2014) investigated the production of β-lactamase in sewage effluents medium by B. subtilis, C. luteola and B. cepacia.
A two-level full factorial design (FFD) was employed to determine the effects of process parameters on lipase production by Candida cylindracea ATCC 14830 in palm oil mill effluent (POME -based medium.
In addition, concern should be given to maintain the same growth rate of Neochloris sp. towards producing high amounts of neutral lipid in effluent based medium.
In order to address this issue, we have attempted to optimize lipase production by Fusarium solani NFCCL 4084 and using palm oil mill effluent (POME) based medium.
The pH of the medium effluent showed no detectable change (pH 7.0 ± 0.2) throughout the experiment.
During continuous culturing the effluent volume of medium and time were monitored and OD600 was measured at regular intervals.
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