In these mice, N-cad was efficiently expressed in the E-cad expression domains.
SDS-PAGE analysis confirmed that the fusion protein HA1-2-fliC was efficiently expressed in an E. coli expression system (Fig. 3a and b).
Unfortunately, the N-terminal thioredoxin fold proved to be insoluble during expression, however the C-terminal α-helical domain (GST-C) was efficiently expressed and purified.
All these semaphorins were relatively efficiently expressed in MDA-MB-231 cells although there were substantial differences in the expression levels obtained with different semaphorins (Fig. 2A).
Though numerous strategy options are available for achieving high expression levels of genes in Escherichia coli, not every gene can be efficiently expressed in this organism.
The enzyme was efficiently expressed heterologously in Trichoderma reesei, purified and biochemically characterized.
Ta0-a was efficiently expressed in the methylotrophic yeast Pichia pastoris strain SMD1168.
All H-DARPin fusion proteins were efficiently expressed on the cell surface.
PL45 was efficiently expressed in Escherichia coli and exhibited remarkable thermal and pH stability.
The fused influenza M2 extracellular region and the HBV core protein can be efficiently expressed in E. coli and spontaneously form VLPs after purification.
The Her2-S-Fab antibody can be efficiently expressed and purified from Escherichia coli, and drive potent cancer cell killing in HER2-overexpressing cancer cells.
