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Compared to P. pastoris, K. lactis as a heterologous expression host, which has been used to efficiently express many proteins in the past few years, and has a GRAS status that permits their use in food and feed applications.
We have engineered an expression vector, p1D4-hrGFP II, in order to efficiently express visual pigments in mammalian cell culture.
Our results revealed that a miRNA-derived loop could be used to efficiently express shRNAs under a CMV promoter and that the expression of the shRNAs could be mediated by a group I intron inserted into the loop region.
The language was intended to make it possible for programmers to efficiently express algorithms.
(F) The primary human T cells efficiently express MSLN-specific CAR, as measured by flow cytometry.
Certainly, we construct the over-complete dictionary Φ c with four-point curve function which can efficiently express the sharp edges and diversity of crack.
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Such studies are not trivial however, because of inherent difficulties in efficiently expressing degenerins using a heterologous expression system.
In these mice, N-cad was efficiently expressed in the E-cad expression domains.
SDS-PAGE analysis confirmed that the fusion protein HA1-2-fliC was efficiently expressed in an E. coli expression system (Fig. 3a and b).
Strikingly, monocistronic vectors, although more efficiently expressed in short term, showed a long term expression lower than that of the bicistronic vector containing the FGF-1 IRES.
Heterologous HIV gag/pol and env genes are more efficiently expressed by FWPV than CNPV in vitro due to longer transgene expression [ 18].
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