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Immunization of HLA-A∗0201 HLA-A∗0201 mice witransgenicnstruct efficiently elicited cellular responses to each intended epitope in vivo, further validating the applicability of this approach.
As before, we observed that only one epitope scaffold combination (ES5) efficiently elicited 2F5 epitope-specific B cells, while two others (ES1 and ES2), although they contained the 2F5 epitope and fixed conformation, poorly elicited 2F5 epitope-specific antibodies.
Expressed I -SceI efficiently elicited recombination in the DR-GFP construct, making 3.7% of the living population GFP positive already after 48 h.
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These data raise the possibility of using specific RTB sub-domains, rather than RTB itself, as antigens to more efficiently elicit neutralizing antibodies and protective immunity against ricin.
The most distinct advantage of mucosal vaccination is that the speedy delivery to mucosal sites efficiently elicits mucosal immune responses, especially the secretion of mucosal IgA (Mcghee et al., 1992).
Intranasal or oral delivery of recombinant HBsAg using this system with CpG as the adjuvant efficiently elicits humoral mucosal immune responses in mice (Borges et al., 2007; Borges et al., 2008).
Taking oligodendrocyte precursors expressing the proteoglycan NG2 (NG2 cells) [26] as a cellular model, we demonstrate that holographic photolysis efficiently elicits glutamatergic currents with fast kinetics and intracellular Ca2+ signals in these cells.
Such recombinant live vaccines have been shown to efficiently elicit both, humoral and cellular immune responses against a variety of heterologous antigens [ 7, 8].
However, in the absence of p53 DRB efficiently elicits apoptosis through an alternative route that may rely on p73 (see below).
Through the intimate collateral connection from the trigeminal sensory pathway to the brainstem 5-HT and NA neuronal systems, the needling of the forehead acupoints with subsequent electrical stimulation could more efficiently elicit afferent acupuncture signals via biophysical and biochemical reactions at local acupoints and, in turn, modulates central 5-HT and NA neuronal functions [ 10].
The director, the Wilma's Czech-born co-artistic director, Jiri Zizka, handles the frequent comings and goings efficiently and elicits an effective performance from Mr. Strathairn as the good-natured but hollow and egoistic Vilem.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com