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The limit of detection was ~11 fg/mL (1.4 fM) while the biosensor probe efficiently distinguished between single-base mismatch and noncomplementary sequence target.
The two parts of the molecular surface which are the contact surface and the reentrant surface can be efficiently distinguished.
Intramembrane protease activity produces a ∼42 kDa MBP fragment which can be identified either by direct staining of SDS-PAGE gels or by blotting using anti-MBP antibodies, and efficiently distinguished from the undigested ∼50 kDa substrate.
Through the comparative analysis with Arabidopsis lipid-related genes, candidate lipid-related genes in the region of QTLs of the Brassica napus can be efficiently distinguished.
While noncharged glycan-engineered cells were efficiently distinguished, the sensor was further tested on cell types with distinct phenotypes, featuring wild-type glycomic signatures.
Despite clear differences in energy metabolism, metastatic prostate cancer cells could not be efficiently distinguished from non-transformed prostate epithelial cells using fluorescent glucose or lipid uptake kinetics.
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The sweep detection was performed using the hapFLK method, which is adapted to analyze whole-genome resequencing data by processing haplotypes instead of single markers, able to efficiently distinguish drift from selection, and efficient for population-related data as well as for divergent lines (Fariello et al. 2013).
Therefore, identifying a biomarker that can efficiently distinguish sepsis from non-infectious SIRS has become an important topic.
As this method clearly and efficiently distinguishes degrees of cold tolerance among cultivars and individuals, it is recommended for gauging cold tolerance in the laboratory.
This study uses reverse transcription-PCR coupled with direct terminator sequencing to economically and efficiently distinguish between sequence types in pooled samples while providing accurate relative quantification.
Both probes can also efficiently distinguish the two MPB-associated fungi from other fungi in the MPB, as well as other related fungi in the order Ophiostomatales.
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