Whereas MKK7α1 displayed no association with any of the WDR62 fragments, MKK7β1 efficiently associated with WDR62 C-terminal fragments Myc-1018-C Myc-1018-C Myc-1018-Cc-1212-C, but not Myc-1018-1284
These artificial SH3 domains efficiently associated with Nef in cells and thereby potently inhibited SH3-dependent Nef functions, such as association with p21-activated kinase-2 and induction of the transcription factor NFAT. On the other hand, biochemical and functional data indicated that the Nef-targeted SH3 domains were not prone to compete with normal SH3-mediated processes.
This indicates that, 40S subunits are efficiently associated to 60S subunits to produce translating ribosomes.
We found that FLAG-tagged VHL-SUMO1ΔC4 is not only efficiently associated with native HA-VHL, but also induced SUMOylation and dimerization of the HA-VHL isoform which is similar in size to the mono SuVHL and oligomerized SuVHL-L protein, respectively (Figure 3G, lane 1).
Using this method, a variety of mammalian MPs were efficiently associated with liposomes co-translationally.
In this study, we demonstrated that a variety of mammalian MPs are efficiently associated with liposomes during wheat cell-free synthesis supplemented with liposomes.
Northern blot analysis confirmed that the control R30 snoRNA efficiently associated with large 90S pre-ribosomes containing the 35S pre-rRNA (upper panel, lanes 13 17).
We found that RIN2 efficiently associated with GFP-tagged active R-Ras 38V, but neither with its dominant-negative mutant (R-Ras 43N) nor with GFP alone.
However, in the absence of masking (dominance coefficient h = 0.5), only FAD can evolve, through efficiently associating itself with genetic backgrounds carrying fewer deleterious mutations.
Conversely, full-length GST-endophilin A1 or a truncated mutant version lacking its BAR domain efficiently associated with soluble recombinant His6-intersectin 1-SH3B (Fig 2F), but not with His6-intersectin 1-SH3A (Fig 2G).
