It was shown that dCas9-VP160 (10 copies of the VP16 minimal activation domain) efficiently activated endogenous genes when targeted by specific sgRNA to the region within 300 bp upstream of the transcriptional start site.
We further showed that while d-HSCs possess most of the multi-lineage long-term self-renewal activity, they are efficiently activated in response to BM injury.
Dormant HSCs were shown to harbor the vast majority of multi-lineage long-term self-renewal activity and to be efficiently activated to self-renew in response to bone marrow injury or granulocyte colony-stimulating factor stimulation.
Whereas BP-2 acted as a full agonist of both ER subtypes, ferutinine was a selective activator of ERα, and α-ZA efficiently activated both receptors with a slight preference for ERα.
Nicotinic acetylcholine receptors (nAChRs), a subset of cholinergic receptors, are fast ionotropic receptors and their activation kinetics suggests that they are efficiently activated by these rapid increases in acetylcholine.
Because ER stress reached a plateau when moderate H2O2 efficiently activated the RISK pathways, we asked whether the activation of RISK pathways limits ER stress development.
TIGAR-hBS2, the more efficient of the two human p53-binding sites, is efficiently activated by either human or mouse p53.
Following chemotactic stimulation, Ras is efficiently activated in pikI− cells, yet Ras-dependent responses (including activation of PKB) are severely impaired.
A relatively small decrease in the SOS-mediated GTP-analog off-rate and consistently an increase in EGF-stimulated activation however suggested that this mutant may be more efficiently activated.
We currently have no explanation for this, but it is tempting to speculate that they are formed prior to activation by UBE1 and represent forms of NEDD8 much more efficiently activated by UBE1.
The modified TGF-β1 produced from hChonJb 7 cells had activity comparable to fully active rhTGF-β1, suggesting that modified TGF-β1 preprotein was more efficiently activated than WT preprotein.
