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Hence, when depleting mice of isolated compartments, tumour protection is not as efficiently abolished as when depleting several immune compartments together.
Blockade of GSK-3β activity by selective inhibitors i.e. lithium or kenpaullone or siRNA-mediated GSK-3β knock-down efficiently abolished these effects.
In addition, when depleting mice of isolated cellular compartments, tumour protection is not as efficiently abolished as when depleting several immune compartments together.
This increase in β-catenin was efficiently abolished when T cells were transfected with siRNA against β-catenin prior to T cell stimulation (Figure 1B).
Similarly, a low concentration of cycloheximide, mimicking the effects of rapamycin on protein synthesis at high glucose, efficiently abolished the ER stress response to glucose and palmitate without affecting global protein synthesis.
Therefore, co-incubation of HepG2 cells with quinones and dicoumarol for 120 minutes efficiently abolished the WST-1 signal (0% and 5% for idebenone and CoQ1, respectively) (Figure 2B).
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Interestingly, the dietary KO supplementation is able to efficiently abolish this effect, keeping the mitochondrial respiratory efficiency unaltered.
Our findings clearly show that MVL-PLA2 efficiently abolishes adhesion and migration of endothelial cells and capillary tube formation.
This indicates that Meis1 knockdown efficiently abolishes CR4.2-GFP expression.
Only a combined preincubation with both 1 μM BQ-123 and 1 μM BQ-788 could efficiently abolish their ET1-induced contraction (Supplementary Movie 8).
These results demonstrated that gene silencing of heparanase can efficiently abolish the proliferation, invasion, metastasis and angiogenesis of human gastric cancer cells in vitro, suggesting that heparanase-specific siRNA is of potential values as a novel therapeutic agent for human gastric cancer.
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