Sentence examples for efficient transposition from inspiring English sources

The Sleeping Beauty (SB) transposon shows efficient transposition and long-term transgene expression in the cells of vertebrates including humans.

High colony numbers in the positive control indicate efficient transposition (Fig. 3B).

The Tol2 transposable element, which was originally found in the medaka fish [ 6], has been reported to be capable of undergoing efficient transposition in a wide variety of vertebrate species including zebrafish, frog, mice, chicken and human [ 7- 12].

Among the DNA transposable elements, the Sleeping Beauty transposon, a member of the mariner family [ 25], has been previously reported to be capable of undergoing efficient transposition in chicken [ 26].

The value of the Tol2 transposon-derived transgenesis strategy has been confirmed by reports of its ability to undergo efficient transposition in a wide variety of vertebrate species [ 7- 12].

Two DNA transposons from non-mammalian species have emerged as gene therapy tools based on their efficient transposition in humans: the reconstructed Tc1/mariner element Sleeping Beauty from salmonid fish and piggyBac from the baculovirus genome [ 95, 96].

All transposon-mediated mutagenesis approaches employ efficient transpositional recombination machinery, which catalyzes the joining of the transposon DNA into target DNA, and the most useful transposition systems integrate into a nearly random selection of target sites [ 24].

Here, we describe the enhanced gene transfer properties of a HIV-1/SB hybrid vector that allows efficient DNA transposition, facilitated by the hyperactive SB100X transposase, from vector DNA intermediates in primary human cells.

Sedlin N functions in endoplasmic reticulum-to-golgi transport while transposase 24 are necessary for efficient DNA transposition.

The transposase was released from Gag in the virus particles in a protease-dependent manner and found to be able to facilitate efficient DNA transposition in transduced cells.

First, the genome may get rid of invading TEs by recombination, but this passive process may turn out to be less efficient than transposition.