Adenoviral vectors seem to be the most efficient transfection method (Mulders et al, 1998).
Magnetofection has already proven to be efficient nonviral transfection method in vitro and in vivo [ 1, 2, 6– 8].
Lu et al. specify the urgent need to develop more efficient delivery nanoparticles and transfection methods in order to apply the nonviral vectors in stem cell engineering and bone regeneration.
The in-vitro experiment indicated that the polyplexes showed efficient transfection efficiency to HEK293T cells.
This fundamental information can typically be derived by in vitro manipulation of gene and protein expression in cell-based assays, approaches that require efficient, simple and reliable cell transfection methods to enable general implementation.
Use of legitimate homologous recombination mechanisms in transfected B. bovis supports the future use of transfection methods for developing efficient gene function assignment experiments using gene knockout techniques.
This method allows for highly efficient transfection of DNA or siRNA into isolated primary cell populations or within mixed cell suspensions as well as into other cell populations that are considered difficult to transfect.
For example, efficient gene targeting has been reported with chemical transfection methods in fibroblasts from sheep [ 14] and pigs [ 36, 37].
However, lipid-based methods commonly used to transfect transformed cells are generally ineffective with primary cells and achieving efficient transfection of primary cells is a common problem in biomedical research.
