La protein is required for the accurate and efficient termination of RNA polymerase III transcription and binds the 3' polyuridine tail of newly synthesised RNAs in the nucleus [23], [24], [25], [26], [27].
Specifically, it appears that both A12.2 and C11 are required for efficient termination of transcription by Pols I and III.
Moreover, the GUS/NAN ratio of p045TMV-NRStop lines was less than 0.02, consistent with efficient termination of NAN translation (Figure 2C).
GRC3 of S.cerevisiae encodes a polynucleotide kinase that is required for efficient termination of transcription by RNA polymerase I.
By virtue of assembling an ECSSOCS1 ligase and interacting with NF-κB subunits, SOCS1 directs ubiquitination and proteasomal degradation of promoter-bound NF-κB members and efficient termination of transcriptional responses (Ryo et al, 2003; Saccani et al, 2004).
This was carried out over a time scale of 3 h up to 48 h polymerization time, enabling the efficient and repeatable termination of the polymerization reactions.
We also restructure the algorithm with efficient termination criteria to achieve the convergence on all the datasets and to improve the reliability of selected set of features.
Our previous chromatin immunoprecipitation experiments demonstrated that mutations in the RNA binding domain of Npl3, such as the npl3-120 aleade, lead to more efficient termination and increased cross-linking of polyA/termination factors to the 3' end of various genes [11].
Significantly, deletion of any part of the minimal required region led to a strong inhibition of BM2fluc synthesis, suggesting that the entire TURBS is required for efficient termination-reinitiation on the segment 7 RNA (Figure 1A).
