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All constructs contain a C-terminal His6-tag followed by two STOP-codons to ensure efficient termination.
Specifically, it appears that both A12.2 and C11 are required for efficient termination of transcription by Pols I and III.
We also restructure the algorithm with efficient termination criteria to achieve the convergence on all the datasets and to improve the reliability of selected set of features.
Ribosomes may also stall at nonsense codons or weak termination codons, each lacking contextual features that elicit efficient termination.
Rho-independent terminators consist of a GC-rich palindromic element followed by a string of U residues, both of which are required for efficient termination.
Moreover, the GUS/NAN ratio of p045TMV-NRStop lines was less than 0.02, consistent with efficient termination of NAN translation (Figure 2C).
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Efficient termination-reinitiation seems to require the close proximity of the stop and start codons [12], [14], [19].
Significantly, deletion of any part of the minimal required region led to a strong inhibition of BM2fluc synthesis, suggesting that the entire TURBS is required for efficient termination-reinitiation on the segment 7 RNA (Figure 1A).
Efficient termination-reinitiation depends upon several factors, including the close proximity of the stop and start codons, the transit of ribosomes along the VP1 mRNA up to the stop codon and a stretch of some 70 80 nucleotides (nt) of mRNA upstream of the stop-start window whose primary sequence, rather than the encoded protein, is key.
However, the capacity of eIF3 to stimulate termination-reinitiation, particularly from a defective TURBS, was confirmed, including a TURBS rendered defective through a point mutation in motif 1. Together, these data support the view that efficient termination-reinitiation requires both mRNA-rRNA interactions and the participation of eIF3.
However, it is possible that some terminators, although incapable of efficient transcription termination, could cause a decrease in the level of transcription.
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