Characterized by tissue specificity and high-efficient specificity targeting at target genes, microRNA may be able to provide a new basis for clinical diagnosis and a new therapeutic approach for some age-related diseases.
Among these are high cleavage efficiency, EGS target specificity, and efficient delivery of the reagent.
Ensuring specificity, efficient delivery to target tissue, and adequate therapeutic effects without toxicity are crucial for the advancement of antiviral gene therapy to clinical use.
MCC is a popular index for measuring the performance of prediction; maximum MCC provides efficient sensitivity and specificity.
Inhibitors of farnesylation that disrupt activation of Ras were not very efficient because of specificity of the transfer of farnesyl/geranyl moiety 48, 49.
For example, siDirect software has been used for the design of siRNA sequences that are highly efficient, with high specificity for the target gene sequence [ 75].
As to non-orthodox R-M systems the absence of recognition site underrepresentation may be explained by a significantly shorter lifespan in bacteria due to efficient mechanisms of specificity changes, or less self-toxicity of such R-M systems.
PCR screening, however, is much more reliable, faster, and efficient with higher specificity owing to effective avoidance of false positive clones identified by repeat sequences in probes by hybridization.
Here, to provide efficient transfer and increased specificity of transgene expression, we synthesized lentiviral vectors (LVs) with myocardial-specific promoters including: α-myosin heavy chain (α-MHC), myosin light chain (MLC2v), and cardiac troponin T (cTnT).
Recent major achievements include significant increases in productivities, yields, and rates of catalytic turnover as well as modification of substrate specificity and efficient multistep reactions in whole-cell biocatalysts.
