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Therefore, our data strongly suggest that the kinase activity of Nol9 is required for efficient rRNA processing.
Interestingly, the mere presence of Nol9 in the cell is not enough to ensure efficient rRNA processing; only the wild-type, kinase-active form of Nol9 is capable to overcome rRNA processing defects upon prior depletion of Nol9.
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This is ideal for understanding the rRNA processing efficiency of individual ribosomal particles.
Point mutations in the C-terminus of rpS14 were shown to delay SSU rRNA 3' processing [29].
To determine whether other rRNA processing steps were affected by DDX21 reduction, we followed rRNA processing for 50 minutes.
It is likely that RNA chaperone activity of CFM4 is needed to maintain precursor-rRNA in processing-competent structures for subsequence rRNA processing.
Originally thought to play a role in rRNA processing due to its nucleolar localization [ 28], nucleoplasmic localization of DDX21 implies functions outside of rRNA processing.
(C and D ) Rps27l loss moderately affects the kinetics of rRNA processing.
NOL9 plays a role in ribosomal RNA (rRNA) processing [ 63] which takes place in the nucleolus.
To test this hypothesis, we investigated the effects of further rm1 mutations on 18S rRNA processing.
In conclusion, the rRNA processing proteins can be disconnected from the rRNA transcription sites indicating that rRNA transcripts are not sufficient to attract the processing proteins in these conditions.
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