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In the paper a novel strategy for efficient proximity detection is presented, which is based on an adoption of dead reckoning.
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Since the emission spectrum of the MLC overlaps the absorption spectrum of the cyanine and both the MLC (the donor) and the cyanine (the acceptor) are in close spatial proximity, efficient resonance energy transfer (RET) does occur.
As the hydride transfer simultaneously exposes a powerful Michael acceptor (ynone or equivalent) and a carbanion in close proximity, efficient annulation might be expected to occur (especially if C is directly attained in situ).
Localization of broad substrate range enzymes and their close proximity for efficient formation of enzyme substrate complex are the significant factors for increasing the efficiency of the cellulosomes.
The importance of maintaining the Pt and Ba phases in close proximity for efficient LNT desulfation was also demonstrated; physically separating the Pt and Ba sites resulted in a shift of the desulfation temperature of the surface BaSO4 by 20 40 °C towards higher temperature, i.e., towards the position characteristic of bulk BaSO4.
The critical issue in the design of an efficient device is the proximity of the biomolecules to the ligands in the capturing stage since the latter is immobilized on the reactive surface and the former is freely moving in the flow.
Initially the stem of the oligonucleotide keeps the fluorophore and quencher in close proximity, promoting efficient quenching.
This close proximity ensures efficient and fast energy transfer.
PC4 helps in bringing the damaged DNA ends in close proximity for efficient ligation and thereby enhances NHEJ.
A previous study has shown that PC4 helps in bridging the DNA ends together and thereby brings them in close proximity for efficient ligation, where it enhances NHEJ and has an important role in DSB repair.
This structure demonstrated that the NADPH FAD binding domain also contained an important linker sequence that could help position the FMN and FAD domains in close proximity for efficient electron transfer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com