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However, an extensive research is still required for the development of new and more efficient pretreatment processes for lignocellulosic feedstocks yielding promising results.
By answering these questions, insights to develop more efficient pretreatment processes and less recalcitrant substrates will be provided.
To date, one of the most significant challenges to biofuel production is the development of economically efficient pretreatment processes.
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Thus, TMP can be considered to be an efficient pretreatment process for AD when methane yield enhancement is a primary concern.
The enhancement of lignocellulose hydrolysis using enzyme complexes requires an efficient pretreatment process to obtain susceptible conditions for the enzyme attack.
The structural stability changes and increased porosity rendered the cellulosic and hemicellulosic contents of the substrate more accessible for hydrolysis hence proving an efficient pretreatment process mandatory.
It could be observed that only a slight increase of 6.5% and 5.5% of the relative absorbance of OCH3 groups after pretreatment using SO2 or CO2, respectively, as expected after an efficient pretreatment process.
Using PCT, important outstanding questions in the area of recalcitrance and deconstruction of biomass can be clarified, which will provide new insights to obtain more efficient substrates and pretreatment processes.
Pretreatment processes allowed efficient COD and G&O reduction using anaerobic treatment; nevertheless, high concentrations of some chemicals from the pretreatment can be harmful for the anaerobic process (Siles et al. 2011).
Therefore, it is necessary to develop and integrate tailor-designed pretreatment processes and efficient microbes that can thrive on many different kinds of biomass.
Determination of the topochemical distribution of lignin and aromatics in sugar cane might also provide important data on the recalcitrance of specific cells, which would be useful for research programs aiming to improve cultivars and design mild pretreatment processes for efficient biomass conversion.
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