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In this paper, an efficient positive feedback source-coupled logic (PFSCL) D latch topology is proposed.
An efficient positive selection method has been optimized for isolation of SAM accumulating yeast strains with significant increases in SAM content on a dry cell weight basis.
ccdB expression thus results in the death of cells containing a non-recombinant vector, offering a highly efficient, positive selection system, even being comparable with white/blue selection systems based on the LacZ operon, one of the most used for this purpose (Bernard 1996; Messing et al. 1977).
However, when mixed populations of transduced and untransduced cells were challenged with HIV-1, the maC46 fusion inhibitor resulted in highly efficient positive selection of transduced cells, an effect that was evident even in mixed populations containing as few as 1% maC46-expressing cells.
A sheath liquid of low pH was used to allow efficient positive ESI.
The allo-specific T cells produced efficient positive control lysis of the EBV cell line used to stimulate the MLR.
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The protocol that we describe here makes use of a commonly available bacterial strain, SW105, and a single selection marker, the galK gene, which allows for very efficient, both positive and negative selection [12], with the negative selection allowing to confirm the flip-out event.
Furthermore, personal history of allergy has been reported to be as efficient as positive skin prick tests to common allergens in detecting associations between atopic diathesis and allergic respiratory diseases in working populations, including animal handlers, bakers, and workers exposed to latex[ 29].
RNA was isolated essentially as described previously [ 26], except that an additional bead beating step was included for efficient Gram-positive cell lysis.
Next to that the IDP pool can be optimized by metabolic pathway engineering, creating a B. subtilis strain as an efficient Gram-positive host for the heterologous production of terpenoids, like the valuable plant-derived pharmaceuticals artemisinin and paclitaxel.
Its upbeat, positive, efficient model of travel gets travelers where they are going in a friendly and comforting way.
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