Sentence examples for efficient miRNA processing from inspiring English sources

This sequence contains the miR-155 stem-loop flanked by portions required for efficient miRNA processing to the mature form.

The miRNA biogenesis pathway is well conserved in diverse organisms and recent data show a requirement of the CBC for efficient miRNA processing and/or activity in plants, mammals, and flies (Gregory et al, 2008; Kim et al, 2008; Laubinger et al, 2008; Gruber et al, 2009; Sabin et al, 2009) (JSY and ECL, unpublished data).

Through in vitro functional study, Noh et al. demonstrated that less efficient miRNA processing caused by knockdown of DROSHA DICER and DGCR8 is responsible for reduced levels of mature forms of tumor-suppressive miRNAs, and finally facilitates breast cancer cell invasion by upregulating the expression of urokinase-type plasminogen activator (uPA) [ 39].

CC smoke exposure, but not THS2.2 aerosol exposure, caused global miRNA downregulation, which may be explained by the interference of CC smoke constituents with the miRNA processing machinery.

Still, the miRNA processing machinery, consisting of DICER-LIKE1 (DCL1) and accessory proteins recognize structural features on the precursors to cleave them at specific places releasing the mature miRNAs.

Our ability to engineer de novo a functional minimal miRNA precursor highlights that we have discovered several elements both necessary and sufficient for accurate miRNA processing.

Furthermore, we showed that hnRNPR exhibits an inhibitory role in miRNA processing while hnRNPH1 functions to facilitate miRNA processing.

In our study we analyzed miRNA processing in HeLa cells on both the pre-miRNA and mature miRNA levels and we tested the effect of over-expression of factors involved in miRNA processing on an array of miRNAs.

Impaired miRNA processing has been found in various tumors [ 10– 13], suggesting a strong connection between miRNA processing and cancer.

Processing of pre-mir-376a2 pre-mir-376a2 pre-mir-376a2hinery produces mature 5′ and 3′ microRNAs.

The expression level of pre-miRNA was calculated as follows: Pre-miR=2−ΔCT (pri-miRNA+pre-miRNA)−2−ΔCT (pri-miRNA) The miRNA processing efficiency is presented as 'relative processing efficiency', which was calculated as the ratio of mature to precursor miRNA.