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More importantly, evidence is accumulating that termination is required for efficient mRNA processing and export, and therefore, for protein expression.
The intercistronic expression element (IEE), shown to direct efficient mRNA processing and promote protein expression [ 28] was incorporated into CEC1 and CEC4 upstream of the aadA gene.
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Lasa, I. et al. Genome-wide antisense transcription drives mRNA processing in bacteria.
In addition to transcriptional regulation, lncRNAs can also control the post-transcriptional mRNA processing such as mRNA splicing and translation.
It is an efficient pre-mRNA processing region that has been widely and successfully used in Trypanosoma cruzi transfection vectors.
SLBP is an architectural protein that is important for efficient pre-mRNA processing of histone mRNAs, histone mRNA export, translation, and degradation of these mRNAs by its ability to interact with the RNA as well as a plethora of proteins in ribonucleoprotein complexes.
Such scenario could explain a less efficient interference in the yeast mRNA processing and packaging reactions with ensuing modest impairment to the cell fitness.
TATA-box genes are also very short and have fewer introns [ 20] therefore their transcription elongation and mRNA processing is more efficient.
The general inhibition of gene expression by inhibition of mRNA processing not only prevents efficient IFN expression but also suppresses the activation of ISGs.
A similar mutation was shown to create a more efficient mRNA cleavage site in the pro-thrombin gene where more efficient processing of the transcript leads to higher pro-thrombin levels and a higher risk of thrombosis [50].
This protein complex not only ensures efficient mRNA cleavage and polyadenylation, but also links 3′end formation to other steps of mRNA biosynthesis and processing (Shi et al, 2009b).
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