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However, efficient loci (η>0.267/40-min) in cds1Δ cells do not show late replication when compared to inefficient loci (η<0.267/40-min η<0.267/40-min
Loci whose ηr was greater that the median ηr-median of ∼0.316 (0.1-min−1) were designated as efficient-replication loci or efficient loci.
On the other hand, the T50 at efficient loci would have wide range from early to late in S-phase (Figure 2E).
Significantly, efficient loci (whose η>0.255/10-min) in wild type cells tend to be replicated late while inefficient ones (whose η<0.255/10-min) early in S-phase (Figure 4).
We defined loci whose replication efficiency ηr was greater than the median ηr-median (or duplication time ΔT was less than the median ΔTmedian) as efficient-replication loci or efficient loci (Figure 2C, loci b, c, and e).
The T50-median of efficient loci in cds1Δ cells was very similar to that of inefficient loci (Figure 7B D), indicating the late efficient-replication origins were most affected by the lack of Cds1p after release from a HU block (Figure 4B D).
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The read length in SLAF-seq ranges between 30 ~ 50 bp, which may provide efficient locus discrimination as compared to that in 2b-RAD (33 ~ 36 bp).
Although reliance on nrITS as the sole source of phylogenetic evidence has come under criticism because of certain features of its evolution [ 9], it remains the most efficient locus for generating species-level phylogenetic inferences in most plant groups.
Segura, V. et al. An efficient multi-locus mixed-model approach for genome-wide association studies in structured populations.
are naturally diploid (2n = 2x = 14) and outbreeding members of the Poaceaea family with a highly efficient two-locus self-incompatibility system.
Therefore, in designing a powerful and efficient multi-locus method for generating testable hypotheses based on genome-wide SNP data, we are motivated to reduce the degrees of freedom.
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