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A highly efficient construct was created by assembling additional copies of DFRsyn and LARsyn enabling a titer of 374.6±43.6 mg/L of -catechin.
The sequence of the most efficient construct TRCN0000008981 was as follows: 5'-CCGGGCTGTGCTTCGTGCCCTACAACTCGAGTTGTAGGGCACGAAG CACAGCTTTTT-3' Infectious viral particles were prepared by co-transfection of 293FT cells with an shRNA expressing plasmid, or a control plasmid, and a lentiviral packaging mix (Virapower K4975-00; Invitrogen) according to the manufacturer's protocol.
We tested 1 ~ 3 siRNAs from Bioneer to select the most efficient construct.
A single EAR copy fused to the truncated MYB80 driven by a strong promoter (for example, B.napus MYB80) proved to be the most efficient construct for obtaining male sterility.
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Green-collar workers are installing solar panels, retrofitting buildings to make them more efficient, constructing transit lines, refining waste oil into biodiesel, erecting wind farms, repairing hybrid cars, building green rooftops, planting trees, and so much more.
The scene graph provides a logical organization for the objects in the 3D world, and allows for efficient constructs and operations such as instancing, hierarchical transforms, nested bounding volumes, and traversals of the scene graph for rendering or collision detection.
The construction of the temporary pavilion is both efficient to construct and affordable by using local materials produced in St . Petersburg.
It is usually more efficient to construct new shop buildings rather than convert old ones to handle modern types of rolling stock.
It is more efficient to construct robust procedures for heavy-tailed innovations, such as the M procedures in Hušková [12, 13] and the references therein.
Efficient centralized control would construct efficient and stable communication links from the control center to every unit.
CC plants were the only plants to show a distinct increase in thermal efficiency based on analysis of decadal data, with less efficient plants constructed before 1990 (∼36% efficient) compared to those that have come on-line since (∼46% efficient).
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