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Hybridization efficiency was validated using interphase nuclei obtained from the bone marrow of a healthy donor, and the thresholds for gains, deletions, and translocations were set at 10%.
An inducer/repressor-free sequential control strategy regulated by glucose concentration in the growth environment was proposed to address these issues, and its efficiency was validated using heterologous beta-carotenoid biosynthesis in Saccharomyces cerevisiae as an example.
Primers efficiency was validated using serial dilutions of cDNA from the SON or PVN.
The Cori cycle's efficiency was validated using flux balance analysis of the integrated multi-tissue model.
siRNA knockdown was performed over a period of 5 days on the mouse primary SFs and efficiency was validated using qRT-PCR to confirm an inhibition of at least 60%.
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The prediction efficiency is validated using past exchange rates not used for training purpose.
The knockdown efficiency of this MO was validated using RT-PCR and qPCR (supplementary material Fig. S3A,B).
The efficiency of the improved approach was validated using an experimental dataset which was recorded by a pair of collocated GPS antenna and an accelerometer, and it shows that the advantages of each sensor are complementary.
The efficiency of this newly developed method was validated using nine trifoliate orange (Poncirus trifoliata) miRNAs predicted computationally.
This assay was validated using an shRNA specific for the GFP sequence (shR-E19) which consistently showed 80% silencing efficiency.
The toolkit was validated using maize protoplasts, transgenic maize lines, and transgenic Arabidopsis lines and was shown to exhibit high efficiency and specificity.
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