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However, the biotransformation efficiency is hampered by the low activity of isoeugenol monooxygenase (IEM) and the product inhibition towards IEM.
Attempts to improve cleavage efficiency were hampered by substantial background activity, which was attributed to inherent affinity between the TEVp fragments.
Particularly for TM proteins, digestion efficiency is hampered by the lack of solubility which results in reduced accessibility of the protein to the protease and sample loss due to precipitation and aggregation.
While the above approach succeeded to create a single cell line expressing GLuc from the endogenous Pmp22 locus, isolation of additional clones was hampered by low efficiency recombination using this reporter insertion strategy.
So I was hampered?
The site was hampered for several hours.
"Well, he played, but he was hampered".
His Belmont was hampered by the start.
I felt he was hampered.
Shooting was hampered by incessant rain.
Furthermore, he was hampered by various injuries.
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