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Transduction efficiency was assessed at day 3 by fluorescence microscopy and flow cytometry.

Depletion efficiency was assessed at 52, 75, and 90 dpi by flow cytometry of tail vein blood (Figure 9B E) and compared to B10Q mice treated with a control Ig (OKM1).

Transduction efficiency was assessed at 2, 7, 14, 21 and 28 days by fluorescence microscopy and flow cytometry.

Quantitative analysis of western blotting revealed that double-siRNA treatment resulted in a more extensive depletion of Ska complex components than single-siRNA treatment, particularly when depletion efficiency was assessed at relatively early time points (Supplementary Figure 5A and B).

The maximum photochemical efficiency was assessed as (FM− F0)/ FM.

Cutting efficiency was assessed by pooling injected embryos at 24 hr post fertilization (hpf), extracting genomic DNA, amplifying the genomic targeted region by PCR (see Supplementary file 1B for primers), and using the Cel1 assay (Oleykowski et al., 1998) to detect evidence of targeted mutations.

Refolding kinetics were monitored fluorometrically over a time period of at least 30 min, and the refolding efficiency was assessed after 24 h incubation under non-denaturing conditions.

The coupling efficiency was assessed spectroscopically by comparing with a predetermined standard curve (by monitoring absorbance at 780 nm).

Transfection efficiency was assessed by Western blot and real-time PCR analysis of nSMase 2 and Atg5 expression at 24 h.

The transduction efficiency was assessed by determining the fluorescence intensity after 4 d of culture.

Plating efficiency was assessed on the second day after the first medium change.