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To investigate spheroids-forming efficiency, sphere propagation assay was carried out on H460 by resuspending single cells into DMEM/F12 supplemented with growth factors.
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In this study, we used the CD133 antigen as a cancer stem-cell marker in order to identify, within stabilized osteosarcoma cell lines, cells exhibiting different properties in terms of proliferation rate, clonogenic efficiency, sphere-cluster formation and dye exclusion.
(b,c) Effects of select pathway inhibitors on colony formation (b), and sphere-forming efficiency and sphere size of basal cells (c).
To test infection efficiency, T24 sphere cells (2 × 10) were infected with indicated adenoviruses at 20 MOI for 6 h and harvested after washing by PBS for three times.
By analyzing Fig. 4 we deduced that the Forward-scattering efficiency of nihility sphere is higher than that of PEC sphere and PMC sphere at higher values of coating thickness and lower at lower values of coating thickness.
In dense urban environments, where UEs move in three dimension (consider, e.g., shopping malls, skyscrapers, and more), it is conceivable that the spectral efficiency per unit sphere might replace the area spectral efficiency as a figure of merit.
Figure 5 reflects that the Back-Scattering efficiency of nihility sphere is lower (but not zero as in uncoated case [12]) than PEC sphere and PMC sphere when coated with DPS metamaterial layer.
The single sphere efficiency of the GAC for the sub-3 nm particles was a power-law of the Peclet number, and the exponent was −0.35.
The single sphere efficiency according to the size of nanoparticles was a function of Peclet number (Pe), and the power-law exponent of Pe was about −0.5.
In Fig. 2, we show the total scattering efficiency for a sphere with different refractive index, where the first Kerker's condition within dipole approximation holds.
By analyzing Fig. 6, we reported that the extinction efficiency of nihility sphere is higher as compared to PEC and PMC cases at higher values of coating thickness and vice versa.
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