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However, only 25 40% of hygromycin-resistant colonies yielded a yellow color, indicating a high rate of spontaneous hygromycin resistance when M. avium is transformed at 32°C, with a final efficiency of transformation of 1 8 × 102 transformants per μg of DNA.
However, the efficiency of transformation obtained was low and transformed lines were difficult to detect due to the low expression of the EGFP marker used.
Our data indicate that DSB mediated by I- SceI in T. reesei improves the efficiency of transformation and the stability of the transformants and promotes gene targeting at a defined locus.
These results contrasted with our earlier observations that transformation of M. avium with non-temperature sensitive plasmids yielded less than 5% of spontaneously resistant colonies and an efficiency of transformation 1.6-log higher (8 × 103 transformants per μg of DNA, [ 12]).
The high sensitivity of the rods by comparison with the cones may be a reflection of the greater concentration in them of pigment that would permit them to catch light more efficiently, or it may depend on other factors e.g., the efficiency of transformation of the light energy into electrical energy.
Although prestin-bait was first transformed using the OHC-pDL2-xN library, the efficiency of transformation was consistently low even after several attempts and few positive clones were identified.
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The combined efficiencies of transformation and homologous recombination (with as few as 450 bp of homology) in C. bescii allow us to use non-replicating plasmids for genetic manipulation.
It is well known that the efficiency of plant transformation mediated by A. tumefaciens varies considerably among plant species [ 4] and that pepper is recalcitrant to transformation [ 44].
Both the threshold detection dose and accuracy of measurements depend on the efficiency of energy transformation.
It has been proven that the efficiency of the transformation in fossil-based cars versus electric cars is 1 3 [7].
To decreasing packet access control delay and improving the efficiency of packet transformation this paper study two methods of ACL designing and application on comparison.
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