Sentence examples for efficiency of the templates from inspiring English sources
However, transposon interruptions in the GAATTC repeats did not increase the in vitro transcription efficiency of the templates.
The rationale of the Q-PCR assay is that the damage in either mitochondrial or nuclear DNA reduces the amplification efficiency of the template, leading to reduction of PCR product with the damaged template.
Furthermore, a novel GT system named in planta GT seems to have effectively overcome problems due to low transformation efficiency of the HR template into plant nuclei, and can adjust the timing of DSB induction at the target locus.
Previous work has shown that the β2-α2 loop forms part of the hypothetical "protein X" epitope which regulates the efficiency of the in vivo template-driven amplification of a prion [44].
This is because the transformation efficiency of the supercoiled vector as template in recombination PCR is more efficient than the nicked recombinant plasmid formed in recombination PCR, which means unacceptable background (false positive) clones would take place without DpnI digestion.
Even if the efficiency of replication of the template is reduced 100-fold due to the mismatch, there would only be a differential amplification of 6-7 cycles between alleles.
The factors influencing the raspberry-like morphology and the deposition efficiency of SiO2 onto templates were discussed, including the surface carboxyl density, TEOS amount and water amount, and the optimal formulation of synthesis was given.
In addition to overall GC content, there was variation in the relationship between the GC content of DG templates and depth of sequencing indicating that the sequence per se, in addition to overall GC content is probably affecting the efficiency of DG template generation and/or bridge amplification on the Illumina GAIIx (Additional file 3).
This approach improved the efficiency of the preparation of the PAA templates remarkably in a normal lab and is expected to be used for the large-scale production in the future.
Together, these data reinforce that the sequences of full-length bypass products are determined by both the efficacy of single nucleotide insertion and the efficiency of bypassed template extension.
