Sentence examples for efficiency of the template from inspiring English sources

The rationale of the Q-PCR assay is that the damage in either mitochondrial or nuclear DNA reduces the amplification efficiency of the template, leading to reduction of PCR product with the damaged template.

The efficiency of the template-blocking PCR method was confirmed by a carefully designed control experiment.

However, transposon interruptions in the GAATTC repeats did not increase the in vitro transcription efficiency of the templates.

Furthermore, a novel GT system named in planta GT seems to have effectively overcome problems due to low transformation efficiency of the HR template into plant nuclei, and can adjust the timing of DSB induction at the target locus.

Even if the efficiency of replication of the template is reduced 100-fold due to the mismatch, there would only be a differential amplification of 6-7 cycles between alleles.

Previous work has shown that the β2-α2 loop forms part of the hypothetical "protein X" epitope which regulates the efficiency of the in vivo template-driven amplification of a prion [44].

In addition to overall GC content, there was variation in the relationship between the GC content of DG templates and depth of sequencing indicating that the sequence per se, in addition to overall GC content is probably affecting the efficiency of DG template generation and/or bridge amplification on the Illumina GAIIx (Additional file 3).

Together, these data reinforce that the sequences of full-length bypass products are determined by both the efficacy of single nucleotide insertion and the efficiency of bypassed template extension.

This is because the transformation efficiency of the supercoiled vector as template in recombination PCR is more efficient than the nicked recombinant plasmid formed in recombination PCR, which means unacceptable background (false positive) clones would take place without DpnI digestion.

The packing efficiency of the model and of its template together with their comparison with the expected value.

For each set of primers and probes, the efficiency of the amplification was assessed using template titrations as recommended by Applied Biosystems.