Sentence examples for efficiency of the siRNA from inspiring English sources

This sequence modification had no effect on the silencing efficiency of the siRNA.

The efficiency of the siRNA knockdown was evaluated by Western blot analysis.

siRNAs used in the screen were designed using an algorithm developed to increase the targeting efficiency of the siRNA while reducing off-target silencing [35].

Infection with CHPV at as low as 36 PFU/ml and as high as 3600 PFU/ml did not alter the efficiency of the siRNA the reduction being 2logs in all cases (Fig 4a-4c).

The efficiency of the siRNA targeting mPIK3CA was confirmed by real-time RT-PCR (mRNA expression of mPIK3CA was knocked down to ∼30% in the siRNA treated cells compared with control cells, data not shown).

To deliver the siRNA in shRNA format and to compare efficiency of the siRNA and shRNA, the sequence of the P-2 siRNA was subjected to the link www.ambion.com/techlib/misc/psilencer_converter.html.html

The knockdown efficiency of the siRNAs was validated by qPCR.

The efficiency of the siRNAs was confirmed by WB.

The knockdown efficiency of the siRNAs was detected by qRT-PCR and western blotting.

To evaluate the knock-down efficiency of the siRNAs, 100 pmol of one of the siRNAs and 1  μg of pCAF-CypA were cotransfected in DF-1 cells.

Knockdown efficiency of the siRNAs were checked using Polymerase Chain Reaction (after reverse transcription) (RT-PCR) with (SPINK1-F 5′-CATCTGCCATATGACCCTTC-3′; SPINK1-R 5′-GATGCCTGTTACCTTCATGG-3′; SPINK1-H 5′-FAM-GACGTGGTAAGTGCGGTGCAG-BHQ1-3′), GAPDH was internal housekeeping control.