The efficiency of the preparations was determined by comparing the amount of viable phage particles from experimental and control groups.
Protein-level calibration utilizing full-length protein as calibrator and full-length SIL-protein as I.S. has demonstrated excellent quantitative performance regardless of the efficiencies of the preparation and digestion procedures, and therefore can be used as the "Gold Standard" for targeted protein quantification.
Comparing with normal electrolysis, the flowing electrolysis showed higher efficiency for the preparation and separation of the products.
When we compared data from technical replicates of 4 runs, the mSPE method provided significantly improved efficiencies for the preparation of samples from urine (e.g., mSPE peptide identification 82% versus 18% with SPE; p = 8.92E-05).
Reliable automatic feedback on any of these parameters will improve the speed and efficiency of the manuscript preparation phase and the review phase for authors, publisher and reviewers.
The extraction efficiency of the sample preparation step before PAH analysis was between 80 and 110%, as determined with reference soil CRM535 (Environmental Research Center, Erembodegem, Belgium).
Kinetic characterisation of the catalytic activity of the different preparations indicated that values of critical monomer concentration (CM) and percentage of cross-linker play an important role in determining the catalytic efficiency of the different preparations.
The plating efficiency of the virus preparations generated in vivo was determined according to the method developed by Hall et al. with minor modifications [ 13] as reported previously [ 17].
By contrast, low concentrations of free Co2+ reduced current efficiency in the preparation of the deposits, while excess concentrations of free Co2+ resulted in cracked films.
