High splicing efficiency was observed for all of the inserted single domain proteins that were used for genetic markers.
To determine the efficiency of DiCre-mediated excision of the inserted Pfama1 genes, we first determined the off-target effect of rapamycin on parasite growth (Fig. S2A C).
Together, these results suggest that the display efficiency is sensitive to the charge of the inserted epitopes.
Together, these results suggest that the display efficiency is sensitive to the charge of the inserted epitope, as both 2xmyc (20-mer) and 3xFLAG (22-mer) are highly charged (50% and 70% charged residues, respectively) whereas the SA-1 peptide (15-mer) is moderately charged, containing only 2 charged residues.
As such, the two empty siRNA cassettes would negatively affect the efficiency of the 4 insert-containing cassettes since the two U6 promoters in the empty cassettes would compete with the four U6 promoters in the insert-containing cassettes for Pol III.
While fluorescence on-plate screening has been available for many years, its adoption has lagged as it depends extensively on the folding efficiency of the gene insert in fusion with the reporter fluorescent protein.
There, Dr. Bing developed a technique that made it possible to measure the mechanical efficiency of the heart by inserting a catheter into a vein in the arm and from there into the main blood vessel that carries deoxygenated blood away from the heart muscle.
To do so, we first measured the catalytic efficiency of PrimPol in inserting the correct nucleotide opposite the four normal template bases.
(A ) Density of small RNAs in sub-telomeric Y′ elements in wild-type and RNA interference (RNAi)+ strains compared with published data for silencing efficiency of inserted transgenes (Pryde and Louis, 1999 ).
Despite the efficiency of inserting a mutation that completely disrupts the function of a gene, most genetic factors in common complex diseases are expected to be noncrucial, coding single nucleotide polymorphisms or expression differences [ 55].
