Sentence examples for efficiency of the cloning from inspiring English sources

Efficiency of the cloning was confirmed by sequencing.

However, in our study microcosms, the recombinant plasmid persisted in the TM cells for 6 years after the introduction, and as the population adapts to the conditions of the microcosms, the efficiency of the cloned gene expression in the cells is restored.

At the same time, the cloning efficiency of the T-A cloning system is greatly reduced when the insert size is increased (Wang et al. 2013a).

The efficiency of cloning the BAC insert into the BGM vector differs between BAC clones used (Additional file 1).

Removal efficiency of the transgenic clone 8 was significantly higher than the wild type hairy roots.

This curve progression of binding efficiency of the individual clones pointed to decisive roles of the inter-domain region and the N-terminal part of the FH1 region for binding to the SH3 domain and the unique site, which is supported by analyzing the shorter clones (S010, S433, S440).

Since two type IIS restriction enzymes have to be used at the same time and since the level 2i-1 destination vector cL2-6 halreadyady a size of 20 kb, we tested again the efficiency of the Golden Gate cloning.

While validation can be very laborious, the increased efficiency of the mapping and cloning steps opens up opportunities to pursue the identification of multiple independent alleles for the same locus.

In addition, the efficiency of seamless cloning methods depends on the intrinsic competency of E. coli cells.

Although apparently healthy animals can be obtained after serial cloning, the efficiency of cloning decreases from generation to generation despite comparable blastocyst and early pregnancy rates.

Additionally, we propose that integration of a physical deficiency map into a high-throughput molecular mapping strategy such as snip-SNP will improve the efficiency of positional cloning in C. elegans.